Victoria's "no jab, no play" laws are being credited with the highest vaccination rates ever recorded in the state.
Health Minister Jill Hennessy said the laws helped to boost the immunisation rate to a 95.3 per cent average across the state.
However the coalition opposition's health spokeswoman Mary Wooldridge said there is a loophole in the no-jab-no-play laws, leaving more than 70,000 children without immunisation.
Parents with health care , concession or Veterans' Affairs cards are exempt, Ms Wooldridge said.
"Victoria is the only jurisdiction with 'no jab' laws who allow an exemption for the concession care card holders," she added.
But the health minister said they are not exempt, but have a grace period of two weeks to allow the most vulnerable children to still access child care and get vaccinated.
"We do have a grace period around the no jab, no play laws to protect those who are particularly vulnerable, like kids that might be in child protection," she said.
Five-year-old getting vaccinated
Highest rates in Melbourne metro (by council area):
Whittlesea - 97.69 per cent
Stonnington - 96.63 per cent
Knox - 96.12 per cent
Melton - 96.17 per cent
Lowest rates in Melbourne metro:
Melbourne - 88.21 per cent
Port Phillip - 92.43 per cent
Greater Dandenong - 92.13 per cent